Beastars: There are strong romantic undertones to Riz's feelings for Tem, even down to the way Riz describes Tem and their relationship, like saying his voice was sweeter than honey and that his eyes were beautiful, and, due to his Self-Serving Memory, picturing and describing eating Tem in an oddly romantic and sexual way.It's not the crossdressing that makes her suspect, but the fact that her charge is a very openly bisexual hedonist who sleeps with most of her servants, male or female - all of which are quite happy with the arrangement.
Later volumes of Baccano! have Carla Alvarez Santonia, unconditionally loyal Bifauxnen bodyguard of Lucrezia de Dormentaire.She tells Osaka that the correct term is 'lesbian,' then says her attraction to Sakaki is 'a teenage thing' adding she wouldn't mind if Sakaki were a guy. The closest the series comes to addressing the issue was when Osaka asked Kaorin if she was gay, in the '10th Anniversary Omnibus'. Azumanga Daioh: The only reason Kaorin's orientation is ambiguous, is because it was never confirmed whether she's actually in love with Sakaki, or simply fangirling really, REALLY, hard over her.
Moe compares them to a married couple, while others tend to see their relationship as platonic. While everyone in the cast knows Mira and Ao are very close, they have different views on the nature of their relationship.